Expressing:Article Title: Anti-CD147 antibody
Article Snippet: .. 1)-12-2 Construction of Human Chimeric and Humanized IgG1-Type Heavy Chain Expression Vector pCMA-G1 A DNA fragment from which the light chain signal sequence and the human κ chain constant region were removed by digesting pCMA-LK with XbaI and PmeI was bound to a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 28 and the DNA sequence encoding the amino acids in the human IgG1 constant region using an In-Fusion HD PCR cloning kit (Clontech Laboratories, Inc.) to construct pCMA-G1. .. 1)-12-3 Construction of Human Chimeric and Humanized IgG2-Type Heavy Chain Expression Vector pCMA-G2 Using a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 29 and the DNA sequence encoding the amino acids in the human IgG2 constant region, pCMA-G2 was constructed in the same manner as in Example 1)-12-2.
Sequencing:Article Title: Anti-CD147 antibody
Article Snippet: .. 1)-12-2 Construction of Human Chimeric and Humanized IgG1-Type Heavy Chain Expression Vector pCMA-G1 A DNA fragment from which the light chain signal sequence and the human κ chain constant region were removed by digesting pCMA-LK with XbaI and PmeI was bound to a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 28 and the DNA sequence encoding the amino acids in the human IgG1 constant region using an In-Fusion HD PCR cloning kit (Clontech Laboratories, Inc.) to construct pCMA-G1. .. 1)-12-3 Construction of Human Chimeric and Humanized IgG2-Type Heavy Chain Expression Vector pCMA-G2 Using a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 29 and the DNA sequence encoding the amino acids in the human IgG2 constant region, pCMA-G2 was constructed in the same manner as in Example 1)-12-2.
Polymerase Chain Reaction:Article Title: Anti-CD147 antibody
Article Snippet: .. 1)-12-2 Construction of Human Chimeric and Humanized IgG1-Type Heavy Chain Expression Vector pCMA-G1 A DNA fragment from which the light chain signal sequence and the human κ chain constant region were removed by digesting pCMA-LK with XbaI and PmeI was bound to a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 28 and the DNA sequence encoding the amino acids in the human IgG1 constant region using an In-Fusion HD PCR cloning kit (Clontech Laboratories, Inc.) to construct pCMA-G1. .. 1)-12-3 Construction of Human Chimeric and Humanized IgG2-Type Heavy Chain Expression Vector pCMA-G2 Using a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 29 and the DNA sequence encoding the amino acids in the human IgG2 constant region, pCMA-G2 was constructed in the same manner as in Example 1)-12-2.
Cloning:Article Title: Anti-CD147 antibody
Article Snippet: .. 1)-12-2 Construction of Human Chimeric and Humanized IgG1-Type Heavy Chain Expression Vector pCMA-G1 A DNA fragment from which the light chain signal sequence and the human κ chain constant region were removed by digesting pCMA-LK with XbaI and PmeI was bound to a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 28 and the DNA sequence encoding the amino acids in the human IgG1 constant region using an In-Fusion HD PCR cloning kit (Clontech Laboratories, Inc.) to construct pCMA-G1. .. 1)-12-3 Construction of Human Chimeric and Humanized IgG2-Type Heavy Chain Expression Vector pCMA-G2 Using a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 29 and the DNA sequence encoding the amino acids in the human IgG2 constant region, pCMA-G2 was constructed in the same manner as in Example 1)-12-2.
Construct:Article Title: Anti-CD147 antibody
Article Snippet: .. 1)-12-2 Construction of Human Chimeric and Humanized IgG1-Type Heavy Chain Expression Vector pCMA-G1 A DNA fragment from which the light chain signal sequence and the human κ chain constant region were removed by digesting pCMA-LK with XbaI and PmeI was bound to a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 28 and the DNA sequence encoding the amino acids in the human IgG1 constant region using an In-Fusion HD PCR cloning kit (Clontech Laboratories, Inc.) to construct pCMA-G1. .. 1)-12-3 Construction of Human Chimeric and Humanized IgG2-Type Heavy Chain Expression Vector pCMA-G2 Using a DNA fragment containing the human heavy chain signal sequence represented by SEQ ID NO: 29 and the DNA sequence encoding the amino acids in the human IgG2 constant region, pCMA-G2 was constructed in the same manner as in Example 1)-12-2.
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